Vlasta Chramostová (17 November 1926 – 6 October 2019) was a Czech film actress. She appeared in 35 films since 1950. She starred in the 1950 film The Trap which was entered into the 1951 Cannes Film Festival. A signatory of Charter 77, she was active in the Velvet Revolution, where the Czechoslovak Socialist Republic was overthrown in November 1989. At a rally at the Vinohrady Theatre in Prague, she was quoted as asking the crowd: "If not now, when? If not us, then who?"

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Vlasta Chramostová was a celebrated Czech film and stage actress trained at the Brno State Conservatory who later became a prominent political dissident.
She is best known for her prominent performances in the psychological horror film The Cremator and the historical drama Sekal Has to Die.
She was a key signatory of Charter 77 and actively participated in the Velvet Revolution.